“I’m bummed, but look forward to seeing Barbie on the big screen,” the star of Trainwreck said at the time.
It was rumoured last January that Anne Hathaway was in talks to take over the role.
In October,it was reported that the film, previously a Sony production, would use a “revised” script by Ocean’s 8 writer Olivia Milch.
The first Barbie doll, made by Mattel, was introduced to the world at the New York Toy Fair in 1959.
The character went on to appear in a string of TV shows and animated films, among them the Toy Story movies.
Scandinavian band Aqua famously had a hit in 1997 with their single Barbie Girl, which contained the lyrics: “Life in plastic, it’s fantastic.”